{"id":24725,"date":"2017-03-31T14:38:35","date_gmt":"2017-03-31T05:38:35","guid":{"rendered":"http:\/\/fserc.kyoto-u.ac.jp\/wp\/?p=24725"},"modified":"2018-02-07T11:20:07","modified_gmt":"2018-02-07T02:20:07","slug":"%e7%92%b0%e5%a2%83dna%e7%a0%94%e7%a9%b6%e3%81%ae%e6%9c%80%e5%89%8d%e7%b7%9a%ef%bc%9a%e6%b5%b7%e6%b0%b4%e3%81%8b%e3%82%89%e9%ad%9a%e3%81%ae%e9%87%8f%e3%82%84%e7%a8%ae%e9%a1%9e%e3%82%92%e7%9f%a5-3","status":"publish","type":"post","link":"https:\/\/fserc.kyoto-u.ac.jp\/wp\/blog\/archives\/24725","title":{"rendered":"\u821e\u9db4\u6c34\u7523\u5b9f\u9a13\u6240\u306b\u304a\u3051\u308b\u74b0\u5883DNA\u8abf\u67fb"},"content":{"rendered":"

\u821e\u9db4\u6c34\u7523\u5b9f\u9a13\u6240\u9577\/\u91cc\u6d77\u751f\u614b\u4fdd\u5168\u5b66\u5206\u91ce \u51c6\u6559\u6388 \u76ca\u7530 \u73b2\u723e<\/p>\n


\n\u25a0\u74b0\u5883DNA\u3068\u306f
\n\u3000\u74b0\u5883DNA\u5206\u6790\u3068\u306f\uff0c\u751f\u7269\u304c\u6c34\u4e2d\u3084\u571f\u58cc\u4e2d\u306b\u6b8b\u3057\u305fDNA\u60c5\u5831\u304b\u3089\uff0c\u751f\u7269\u306e\u5728\u4e0d\u5728\u3084\u751f\u7269\u91cf\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u6280\u6cd5\u3067\u3042\u308b\u3002\u901a\u5e38\uff0c\u6570\u5341mL\u304b\u3089\u6570L\u306e\u74b0\u5883\u6c34\u3092\u63a1\u53d6\u3057\u3066\u30d5\u30a3\u30eb\u30bf\u30fc\u3067\u6ffe\u904e\u3057\uff0c\u3053\u306e\u30d5\u30a3\u30eb\u30bf\u30fc\u304b\u3089DNA\u3092\u62bd\u51fa\u3059\u308b\u3002\u5b9a\u91cf\u6027\u3092\u91cd\u8996\u3059\u308b\u5834\u5408\uff0c\u5bfe\u8c61\u7a2e\u306b\u7279\u7570\u7684\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066\u305d\u306e\u751f\u7269\u306eDNA\u3092PCR\u3067\u5897\u5e45\u3057\u5b9a\u91cf\u3092\u884c\u3046\u3002\u307e\u305f\u591a\u69d8\u6027\u306b\u3064\u3044\u3066\u8abf\u3079\u305f\u3044\u5834\u5408\uff0c\u30e6\u30cb\u30d0\u30fc\u30b5\u30eb\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066DNA\u3092\u5897\u5e45\u3057\uff0c\u65e2\u5b58\u306e\u907a\u4f1d\u5b50\u914d\u5217\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3092\u53c2\u7167\u3057\u3066\u751f\u606f\u7a2e\u3092\u7279\u5b9a\u3059\u308b\u3002
\n\u3000\u821e\u9db4\u6c34\u7523\u5b9f\u9a13\u6240\u3067\u306f\uff0c2013\u5e74\u5ea6\u304b\u3089\u79d1\u5b66\u6280\u8853\u632f\u8208\u6a5f\u69cb\u306b\u3088\u308b\u6226\u7565\u7684\u5275\u9020\u7814\u7a76\u63a8\u9032\u4e8b\u696d(CREST)\u306e\u652f\u63f4\u3092\u53d7\u3051\uff0c\u300c\u74b0\u5883DNA\u5206\u6790\u306b\u57fa\u3065\u304f\u9b5a\u985e\u7fa4\u96c6\u306e\u5b9a\u91cf\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3068\u751f\u614b\u7cfb\u8a55\u4fa1\u624b\u6cd5\u306e\u958b\u767a\u300d\uff08\u4ee3\u8868\uff1a\u9f8d\u8c37\u5927\u5b66\u30fb\u8fd1\u85e4\u502b\u751f\u6559\u6388\uff09\u306e\u4e00\u7ffc\u3092\u62c5\u3063\u3066\u3044\u308b\u3002\u3053\u306e\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3067\u306f\uff0c\u74b0\u5883DNA\u5206\u6790\u3092\u6d77\u57df\u306b\u5c55\u958b\u3057\u9b5a\u985e\u306e\u751f\u7269\u91cf\u3092\u5b9a\u91cf\u3059\u308b\u6280\u8853\u306e\u78ba\u7acb\u3092\u76ee\u6307\u3057\u3066\u3044\u308b\u3002\u6c34\u69fd\u5b9f\u9a13\u304a\u3088\u3073\u30d5\u30a3\u30fc\u30eb\u30c9\u8abf\u67fb\u3092\u901a\u3057\u3066\uff0c\u821e\u9db4\u6c34\u7523\u5b9f\u9a13\u6240\u304c\u672c\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u306b\u304a\u3044\u3066\u679c\u305f\u3057\u3066\u304d\u305f\u5f79\u5272\u3092\u6982\u8aac\u3059\u308b\u3002
\n\u25a0\u98fc\u80b2\u5b9f\u9a13
\n\u3000\u6c34\u69fd\u5185\u306b\u30de\u30a2\u30b8\u30921\uff0c3\uff0c10\u307e\u305f\u306f30\u5c3e\u53ce\u5bb9\u3057\uff0c\u98fc\u80b2\u6c34\u4e2d\u306eDNA\u3092\u5b9a\u91cf\u3057\u305f\u3068\u3053\u308d\uff0c\u9b5a\u306e\u6570\u304c\u5897\u3048\u308b\u306b\u3057\u305f\u304c\u3063\u3066DNA\u306e\u691c\u51fa\u91cf\u3082\u76f4\u7dda\u7684\u306b\u5897\u3048\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u307e\u305f\u4e00\u65e5\u306e\u3046\u3061\u7570\u306a\u308b\u6642\u9593\u5e2f\u3067\u63a1\u6c34\u3057\u305f\u5834\u5408\uff0c\u691c\u51fa\u3055\u308c\u308bDNA\u91cf\u306b\u9055\u3044\u306f\u306a\u304b\u3063\u305f\u3002
\n\u3000\u7d9a\u3044\u3066\uff0c\u7570\u306a\u308b\u9577\u3055\u306e2\u7a2e\u985e\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066\u30de\u30a2\u30b8\u306eDNA\u306e\u691c\u51fa\u3092\u8a66\u307f\u305f\u3068\u3053\u308d\uff0c\u9577\u3044\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u691c\u51fa\u3055\u308c\u308b\u9577\u9396\u306eDNA\u306f\u52a3\u5316\u304c\u901f\u3044\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u3053\u306e\u3053\u3068\u3092\u5229\u7528\u3057\u3066\uff0cDNA\u306e\u653e\u51fa\u5f8c\u306e\u76f8\u5bfe\u7684\u306a\u6642\u9593\u60c5\u5831\u306b\u3064\u3044\u3066\u3082\u74b0\u5883DNA\u5206\u6790\u304b\u3089\u5f97\u3089\u308c\u308b\u53ef\u80fd\u6027\u304c\u62d3\u304b\u308c\u305f(Jo et al. 2017)\u3002\u672c\u7814\u7a76\u306e\u5b9f\u9a13\u3092\u62c5\u5f53\u3057\u305f\u795e\u6238\u5927\u5b66\u306e\u5f90\u5bff\u660e\u3055\u3093\u306f\uff0c\u7b2c63\u56de\u65e5\u672c\u751f\u614b\u5b66\u4f1a\u5927\u4f1a\u306b\u304a\u3044\u3066\uff0c\u300c\u74b0\u5883DNA\u306e\u65ad\u7247\u9577\u306b\u3088\u308b\u898b\u305f\u76ee\u306e\u5206\u89e3\u901f\u5ea6\u306e\u9055\u3044\u300d\u3068\u984c\u3057\u3066\u5f53\u5b9f\u9a13\u6240\u306e\u6559\u54e1\u304a\u3088\u3073\u5927\u5b66\u9662\u751f\u3068\u306e\u5171\u8457\u3067\u6210\u679c\u767a\u8868\u3057\uff0c\u540c\u5927\u4f1a\u751f\u7269\u591a\u69d8\u6027\u5206\u91ce\u306e\u6700\u512a\u79c0\u30dd\u30b9\u30bf\u30fc\u8cde\u3092\u53d7\u8cde\u3057\u305f\u3002
\n\u25a0\u821e\u9db4\u6e7e\u306b\u304a\u3051\u308b\u30de\u30a2\u30b8\u306e\u5b9a\u91cf
\n\u3000\u821e\u9db4\u6e7e\u5185\u306e\u5e83\u57df100\u5730\u70b9\u306b\u304a\u3044\u3066\uff0c\u8868\u5c64\u3068\u5e95\u5c64\u3067\u63a1\u6c34\u3092\u884c\u3044\uff0c\u3053\u308c\u3089\u304b\u3089DNA\u3092\u62bd\u51fa\u3057\uff0c\u30de\u30a2\u30b8\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066\u672c\u7a2e\u306eDNA\u3092\u5b9a\u91cf\u3057\u305f\u3002\u540c\u6642\u306b\u8a08\u91cf\u9b5a\u7fa4\u63a2\u77e5\u6a5f\u306b\u3088\u3063\u3066\u5404\u5730\u70b9\u306b\u304a\u3051\u308b\u30de\u30a2\u30b8\u306e\u5bc6\u5ea6\u3092\u63a8\u5b9a\u3057\u305f\u3002\u4e21\u8005\u3092\u6bd4\u8f03\u3057\u305f\u3068\u3053\u308d\uff0c\u9b5a\u63a2\u3067\u30de\u30a2\u30b8\u304c\u591a\u304f\u691c\u77e5\u3055\u308c\u305f\u5730\u70b9\u3067\u306f\u30de\u30a2\u30b8\u306eDNA\u304c\u591a\u304f\u691c\u51fa\u3055\u308c\u308b\u50be\u5411\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f (Yamamoto et al.2016)\u3002
\n\u25a0\u821e\u9db4\u6e7e\u306b\u304a\u3051\u308b\u9b5a\u985e\u7fa4\u96c6
\n\u3000\u4e0a\u8ff0\u306e\u5e83\u57df\u8abf\u67fb\u306b\u304a\u3044\u3066\u62bd\u51fa\u3057\u305fDNA\u306b\u3064\u3044\u3066\uff0c\u30e6\u30cb\u30d0\u30fc\u30b5\u30eb\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u305f\u9b5a\u985e\u7fa4\u96c6\u306e\u63a8\u5b9a\u3092\u8a66\u307f\u305f\u3002\u3053\u308c\u306b\u3088\u308a\uff0c\u6de1\u6c34\u9b5a\u3092\u542b\u3080128\u9b5a\u7a2e\u3092\u691c\u51fa\u3057\u305f\u3002\u3053\u306e\u9b5a\u7a2e\u30ea\u30b9\u30c8\u3067\u306f\uff0c\u821e\u9db4\u6e7e\u5185\u3067\u904e\u53bb14\u5e74\u9593\u884c\u3063\u3066\u304d\u305f\u6f5c\u6c34\u76ee\u8996\u8abf\u67fb\u306e\u51fa\u73fe\u9b5a\u7a2e\u306e\u3046\u3061\uff0c\u51fa\u73fe\u983b\u5ea6\u304c\u4e0a\u4f4d\u3068\u306a\u308b\u3082\u306e\u306e8\u5272\u8fd1\u304f\u3092\u691c\u51fa\u3067\u304d\u3066\u3044\u308b (Yamamoto et al. 2017)\u3002\u672c\u6210\u679c\u306b\u3064\u3044\u3066\u306f\uff0c\u4eac\u90fd\u5927\u5b66\u30fb\u795e\u6238\u5927\u5b66\u30fb\u9f8d\u8c37\u5927\u5b66\u30fb\u5317\u6d77\u9053\u5927\u5b66\u306e\u7814\u7a76\u8005\u3089\u3068\u306e\u5408\u540c\u8a18\u8005\u767a\u8868\u30922017\u5e741\u670811\u65e5\u306b\u884c\u3063\u305f\u3002
\n\u25a0\u30af\u30e9\u30b2\u306e\u74b0\u5883DNA
\n\u3000\u30af\u30e9\u30b2\u3082\u307e\u305f\u6d77\u6c34\u4e2d\u306b\u591a\u91cf\u306eDNA\u3092\u653e\u51fa\u3059\u308b\u3053\u3068\u304c\uff0c\u30a2\u30ab\u30af\u30e9\u30b2\u3092\u7528\u3044\u305f\u98fc\u80b2\u5b9f\u9a13\u306b\u3088\u308a\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u307e\u305f\u821e\u9db4\u6c34\u7523\u5b9f\u9a13\u6240\u306e\u685f\u6a4b\u3067\u63a1\u6c34\u3055\u308c\u305f\u6d77\u6c34\u4e2d\u306e\u30a2\u30ab\u30af\u30e9\u30b2DNA\u91cf\u306f\uff0c\u3053\u306e\u685f\u6a4b\u304b\u3089\u6bce\u671d\u8a18\u9332\u3057\u3066\u3044\u308b\u30a2\u30ab\u30af\u30e9\u30b2\u306e\u500b\u4f53\u6570\u306e\u5909\u52d5\u3068\u5927\u5909\u3088\u304f\u4e00\u81f4\u3057\u305f(Minamoto et al. 2017)\u3002\u3053\u306e\u7814\u7a76\u306f\uff0c\u5b9f\u9a13\u3092\u62c5\u5f53\u3057\u305f\u795e\u6238\u5927\u5b66\u306e\u798f\u7530\u5411\u82b3\u3055\u3093\u304c\u7b2c62\u56de\u306b\u672c\u751f\u614b\u5b66\u4f1a\u3067\u30dd\u30b9\u30bf\u30fc\u767a\u8868\u3057\u305f\u969b\uff0c\u4fdd\u5168\u5206\u91ce\u306e\u6700\u512a\u79c0\u30dd\u30b9\u30bf\u30fc\u8cde\u3092\u53d7\u8cde\u3057\u3066\u3044\u308b\u3002
\n\u25a0\u74b0\u5883DNA\u5206\u6790\u306e\u6301\u3064\u30dd\u30c6\u30f3\u30b7\u30e3\u30eb
\n\u3000\u74b0\u5883DNA\u5206\u6790\u306f\uff0c\u751f\u606f\u3059\u308b\u751f\u7269\u3092\u640d\u306a\u3046\u3053\u3068\u306a\u304f\uff0c\u9ad8\u3044\u611f\u5ea6\u3067\u5728\u4e0d\u5728\u3084\u304a\u304a\u307e\u304b\u306a\u751f\u7269\u91cf\u306b\u95a2\u3059\u308b\u60c5\u5831\u3092\u53d6\u5f97\u3059\u308b\u3053\u3068\u306e\u3067\u304d\u308b\u624b\u6cd5\u3067\u3042\u308b\u3002\u4eca\u5f8c\u306f\u74b0\u5883DNA\u5206\u6790\u3092\u3088\u308a\u5e83\u57df\u3042\u308b\u3044\u306f\u9577\u671f\u306e\u8a66\u6599\u306b\u9069\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\uff0c\u751f\u614b\u5b66\u306e\u7814\u7a76\u8005\u304c\u304b\u3064\u3066\u624b\u306b\u3057\u305f\u3053\u3068\u306e\u306a\u3044\u9769\u547d\u7684\u306a\u8cea\u3068\u91cf\u306e\u30c7\u30fc\u30bf\u304c\u5f97\u3089\u308c\u308b\u3067\u3042\u308d\u3046\u3002
\n\u73fe\u5728\uff0c\u69d8\u3005\u306a\u7121\u810a\u690e\u52d5\u7269\u3084\u690d\u7269\u306b\u9069\u7528\u53ef\u80fd\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u958b\u767a\u3055\u308c\u3064\u3064\u3042\u308b\u3002\u9069\u5207\u306a\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u3092\u884c\u3048\u3070\uff0c\u62bd\u51fa\u3057\u305fDNA\u306f\u69d8\u3005\u306a\u7528\u9014\u3078\u3068\u5229\u7528\u304c\u53ef\u80fd\u3067\u3042\u308b\u3002\u591a\u69d8\u306a\u5206\u985e\u7fa4\u3092\u5bfe\u8c61\u3068\u3059\u308b\u68ee\u91cc\u6d77\u9023\u74b0\u306e\u7814\u7a76\u306b\u304a\u3044\u3066\u3082\uff0c\u753b\u671f\u7684\u306a\u30c4\u30fc\u30eb\u306b\u306a\u308b\u3082\u306e\u3068\u671f\u5f85\u3067\u304d\u308b\u3002<\/p>\n

Jo T, Murakami H, Masuda R, Sakata M, Yamamoto S, Minamoto T (2017). Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA. Molecular Ecology Resources 17(6): e25-e33.
\nhttps:\/\/doi.org\/10.1111\/1755- 0998.12685
\nMinamoto T, Fukuda M, Katsuhara KR, Fujiwara A, Hidaka S, Yamamoto S, Takahashi K, Masuda R (2017). Environmental DNA reflects spatial and temporal jellyfish distribution. PLoS ONE 12(2): e0173073\uff0e
\nYamamoto S, Minami K, Fukaya K, Takahashi K, Sawada H, Murakami H, Tsuji S, Hashizume H, Kubonaga S, Horiuchi T,Hongo M, Nishida J, Okugawa Y, Fujiwara A, Fukuda M, Hidaka S, Suzuki KW, Miya M, Araki H, Yamanaka H, Maruyama A, Miyashita K, Masuda R, Minamoto T, Kondoh M (2016).Environmental DNA as a \u2018snapshot\u2019 of fish distribution: A case study of Japanese jack mackerel in Maizuru Bay, Sea of Japan. PLoS ONE 11: e0149786.
\nYamamoto S, Masuda R, Sato Y, Sado T, Araki H, Kondoh M, Minamoto T, Miya M (2017). Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea. Scientific Reports 7: 40368.
\n

\n\n
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\n\"\"\n<\/a>

\u7dd1\u6d0b\u4e38\u304b\u3089\u3072\u3057\u3083\u304f\u3092\u7528\u3044\u3066\u306e
\n\u8868\u5c64\u6c34\u306e\u63a1\u6c34\uff08\u821e\u9db4\u6e7e\u5185\uff09
\n<\/p><\/div><\/td>

\n
\n\"\"\n<\/a>

\u98fc\u80b2\u5b9f\u9a13\u6c34\u69fd\u304b\u3089\u63a1\u6c34\u3059\u308b\u4eac\u5927\u30fb\u795e\u6238\u5927\u304a\u3088\u3073
\n\u9f8d\u8c37\u5927\u306e\u9662\u751f\u30fb\u5b66\u90e8\u751f\uff08\u821e\u9db4\u6c34\u7523\u5b9f\u9a13\u6240\u98fc\u80b2\u68df\uff09
\n<\/p><\/div><\/td><\/tr><\/table><\/div>\n

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